Passion Standard Poodles has two outstanding champion studs available at this time through artificial insemination. Stud service is available to approved bitches only.

Frozen semen can be done over great distance without having to send your bitch to the stud. Please check for more information on each available stud.

Please check back as more studs will be available in the near future once genetic testing is completed.

FROM: COLORADO INTERNATIONAL CANINE SEMEN BANK

Frozen Semen Breeding:

If you are arranging a frozen semen breeding there are a few important things to keep in mind. You want to be sure that you fully understand the costs involved.

You and the stud owner/semen owner should agree upon the stud fee. Brighton Animal Clinic and ICSB-CO are not involved in these arrangements.

As the bitch owner, you will be expected to pay for several things in addition to the stud fee. Generally there is a charge for the release of the frozen semen from the storage facility, rental of the shipping dewar, and shipping charges for overnight delivery to the veterinarian that will be doing the insemination for you.

You should ensure that the veterinarian performing the insemination for you is well versed in the procedure and comfortable with ovulation timing and using frozen semen. You also need to completely understand the method of insemination used by the veterinarian. Most commonly, frozen semen is implanted using surgical insemination. Other methods include trancervical via a rigid pipette or a guided trancervical via endoscope. Vaginal inseminations with frozen semen have proven to be successful, however, not felt to acquire normal sized litters.

With the aid of ovulation timing, the veterinarian can plan the frozen semen breeding for you to generally occur on D5 post-LH surge, or at progesterone levels of 15-20 ng/ml.

At Brighton Animal Clinic and ICSB-CO, our conception rate with frozen semen breeding has been consistently 85-89% for the last 5 years. Our method of insemination is generally surgical insemination. We have had success with vaginally inseminating frozen semen in an Australian Cattle Dog: 5 pups, Airedale Terrier: 5 pups, Welsh Pembroke Corgi: 4 pups, Golden Retriever: 9 pups, and another Airedale Terrier: 10 pups.

Our largest frozen semen breeding has been in 2 different bitches. An Irish Setter bitch conceived 11 pups, delivered 10 live pups and a Small Munsterlander bitch conceived and delivered 11 pups.

OVULATION TIMING

The ovulation timing of the bitch is probably the most important aspect to a successful breeding, regardless of whether you use natural breeding, planned AI’s, chilled semen, or frozen semen.

Improved conception rates are recognized when breeding naturally if one will ovulation time the bitch and ensure that the breeding occurs during her most fertile period.

Ovulation timing involves several entities.

• Knowing when the bitch comes in to season, d1 (day 1).
• Progesterone testing beginning d5 (day 5) of estrous.
• LH testing if necessary and recommended for frozen semen breeding.
• Vaginal cytology used to corroborate the progesterone results and physical signs.

Also, most helpful to determine the day of diestrous and help predict the day of delivery.
The most commonly used and understood entity is the progesterone test.

We recommend that you start having progesterone tests run on your bitch beginning d5 of her season. These tests are blood tests that are drawn from your bitch and sent to a laboratory to be run and a quantitative result is reported in nanograms/ml. This result helps to estimate when the LH surge happens, and therefore, helps to determine when the bitch’s most fertile period is for breeding.

The progesterone tests should be run every other day to have enough data to determine when the LH surge occurs. When progesterone value doubles or is at least 1.5– 2.5 ng/ml, this should coincide with the LH surge.

Research shows that bitches will ovulate between 48 and 72 hours after LH surge. The ovulated eggs are not capable of being fertilized until 60 hours after ovulation. The eggs are receptive then for 48 hours.

When the date of LH surge is determined, consider that DØ. Then count forward 4 days and that would be the first day for breeding naturally or with chilled semen. Usually progesterone level is near 8-11 ng/ml on the first day of breeding.

Frozen semen breeding should be implanted at progesterone levels of 15-20 ng/ml.
(Click To View PDF file chart)

LH testing is recommended when planning frozen semen breeding or when breeding a bitch that has history of difficulty of conceiving. LH tests must be done daily and blood drawn at approximately the same time of day each time. LH is only measurable in the bitch for a short time, 18 to 24 hours. If the blood is not drawn at the same time of day, the presence of LH can be missed. An in-house test kit for LH is available from Synbiotics and is more reliable than their Pro-Spot test kit.

The best way to save on number of tests run is to draw the blood daily when a progesterone level close to 1.0 ng/ml is reached. Save serum from the “off” days as well as the days that you continue to run progesterone levels. Then you can go back and run LH on the saved serum after the rise in the progesterone level has indicated the surge has happened. With this test kit, a positive test result indicates the presence of LH and you definitively identify the LH surge.

For further questions please contact the ICSB at: Brighton Animal Clinic, 180 E. Bromley Lane, Brighton CO 80601 (303)-659-2472 Fax: (303) 637-7697
Email: BrightonAClinic@aol.com